Cluster A phages most commonly have siphoviridae morphotypes and are classified as temperate phages. Since our phage is lytic this could imply that our phage has its repressor gene deleted but we would need to sequence the entire genome to be sure.
This was really well-presented research. I understood every word and it made so much sense, thank you! What is the significance of which cluster the phage belongs to?
Hi! One advantage of our phage as being classified as lytic is that we know that our phage reproduces immediately and then lyses the host cell. This would make our phage useful for killing unwanted bacteria. For example, if we knew that there was a pathogenic bacteria on some lettuce produce we would want to use a lytic cell that infects that specific bacteria because it would most effectively kill the pathogenic bacteria
Knowing the cluster can tell insight about your phages genome. Additionally, grouping phages allows researchers to more easily select many phages that are closely related and test them with a bacteria for gene therapy.
I did not see the question above. Do you think you could elaborate on the advantages or disadvantages that your phage would have being classified as lytic?
Hello, I think you did an excellent job walking us through your research from beginning to end. Could you elaborate on your future directions past the confirmation of your findings through PCR or sequencing?
So once we have confirmed our phages clustering we would like to examine its genome to potentially characterize and understand the exact functionality and layout of our phages genome.
In PCR you mix your DNA with the hypothesized cluster enzymes. Then you run your samples on the gel along with a positive and negative control to see if there has been any amplification. If there has been amplification by cluster A then we know our phage belongs in cluster A. If not we would need to do another round of PCR with different cluster enzymes.
Hi! What would be the significance of the bacteriophage belonging to cluster A?
Cluster A phages most commonly have siphoviridae morphotypes and are classified as temperate phages. Since our phage is lytic this could imply that our phage has its repressor gene deleted but we would need to sequence the entire genome to be sure.
This was really well-presented research. I understood every word and it made so much sense, thank you! What is the significance of which cluster the phage belongs to?
I did not see the question above. Do you think you could elaborate on the benefits or adversities your phage has being classified as lytic?
Hi! One advantage of our phage as being classified as lytic is that we know that our phage reproduces immediately and then lyses the host cell. This would make our phage useful for killing unwanted bacteria. For example, if we knew that there was a pathogenic bacteria on some lettuce produce we would want to use a lytic cell that infects that specific bacteria because it would most effectively kill the pathogenic bacteria
Knowing the cluster can tell insight about your phages genome. Additionally, grouping phages allows researchers to more easily select many phages that are closely related and test them with a bacteria for gene therapy.
I did not see the question above. Do you think you could elaborate on the advantages or disadvantages that your phage would have being classified as lytic?
Hello, I think you did an excellent job walking us through your research from beginning to end. Could you elaborate on your future directions past the confirmation of your findings through PCR or sequencing?
So once we have confirmed our phages clustering we would like to examine its genome to potentially characterize and understand the exact functionality and layout of our phages genome.
You mentioned using a PCR for future directions, how would using a PCR help to determine is your phage belonged
to the cluster described?
In PCR you mix your DNA with the hypothesized cluster enzymes. Then you run your samples on the gel along with a positive and negative control to see if there has been any amplification. If there has been amplification by cluster A then we know our phage belongs in cluster A. If not we would need to do another round of PCR with different cluster enzymes.