8 thoughts on “P89 – Grimison

    1. It could have been a really low concentration of DNA in the sample that did not produce enough for the gel to show anything, or the DNA did not unbind from the beads that were used in DNA isolation well.

  1. Good presentation! I took this lab but forget which lifecycle is better at killing bacteria?

    1. Thanks! Lytic phages undergo only the lytic lifecycle where the cell is lysed ending in bacterial death so they would kill the most, compared to temperate phage that is a mix of lytic and lysogenic phage.

  2. Great presentation! Can you elaborate on the experiments you were unable to do due to limitations? Why would you have conducted those experiments and what did you expect to see?

    1. We were unable to do restriction digest and PCR of our phage’s DNA. Without restriction digest we were unable to make a phage cluster prediction that implies they likely share around 50% of their DNA. PCR would also act as a way of verifying this cluster prediction but sequencing would need to be done to say for certain. We didn’t really expect much as restriction digest is where we would make predictions from.

  3. Good job! How can you tell the tail is non-contractile by looking at it? How does this differ from a contractile tail?

    1. Thanks! Generally you can tell is not contractile because it will have a longer (over around 120 nm), skinnier appearance. A contractile tell is shorter and generally wider, and this could also be identified based on Electron microscopy detail.

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