18 thoughts on “P157 – Francone

    1. I wish that I was able to have run PCR on Chino to me it would have been important to identify what cluster Chino was from

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    2. I wish that I would be able to run PCR and see the DNA cluster that the phage belonged to. I would also like to see if this phage helps with various to foreign DNA. Which means that it could help cells fight off different diseases

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  1. How could you overcome the dormancy of the phage in long term goals of using this phage in antibiotic resistant infections?

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    1. Yes there are two types of proteins that have been shown that can force a temperate phage into a lytic phage life cycle. With the removal of these proteins the temperate phage will only move into the lytic cycle

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  2. How can this research be used towards the goal of using the phage in antibiotic resistant infections?

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    1. Chino could be important in identifying a virus that it can target. A temperate phage can have proteins remove that will make it only lystate and make it more effective. Chino could help with important disease

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  3. Awesome work! As I was listening to your presentation I was wondering if the structure and shape of your phage allowed it to infect cells in a specific way or could be used to explain any of the results that you obtained.

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    1. A siphoviridae phage will have a longer tail this means that it has a more gentle approach when it integrates itself into the host cells DNA. This tail allows it to not force it self in but rather inject itself

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    2. A siphoviridae phage will have a longer tail this means that it has a more gentle approach when it integrates itself into the host cells DNA. This tail allows it to not force it self in but rather inject itself.

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    3. A siphoviridae phage will have a longer tail this means that it has a more gentle approach when it integrates itself into the host cells DNA. This tail allows it to not force it self in but rather inject itself

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    1. The first time that we ran dna isolation we didn’t have a high quality gel it was only high in quantity. So the DNA isolation had to be run again. If I had more time I would want to take the second DNA gel and go head and use to PCR cluster. I just wish that I had more time

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