18 thoughts on “P157 – Francone

  1. Is there anything you had wished to be able to do more of in your research?

    1. I wish that I was able to have run PCR on Chino to me it would have been important to identify what cluster Chino was from

    2. I wish I was able to run PCR and verify what cluster chino was apart of

    3. I wish that I would be able to run PCR and see the DNA cluster that the phage belonged to. I would also like to see if this phage helps with various to foreign DNA. Which means that it could help cells fight off different diseases

  2. How could you overcome the dormancy of the phage in long term goals of using this phage in antibiotic resistant infections?

    1. Yes there are two types of proteins that have been shown that can force a temperate phage into a lytic phage life cycle. With the removal of these proteins the temperate phage will only move into the lytic cycle

  3. How can this research be used towards the goal of using the phage in antibiotic resistant infections?

    1. Chino could be important in identifying a virus that it can target. A temperate phage can have proteins remove that will make it only lystate and make it more effective. Chino could help with important disease

  4. Awesome work! As I was listening to your presentation I was wondering if the structure and shape of your phage allowed it to infect cells in a specific way or could be used to explain any of the results that you obtained.

    1. A siphoviridae phage will have a longer tail this means that it has a more gentle approach when it integrates itself into the host cells DNA. This tail allows it to not force it self in but rather inject itself

    2. A siphoviridae phage will have a longer tail this means that it has a more gentle approach when it integrates itself into the host cells DNA. This tail allows it to not force it self in but rather inject itself.

    3. A siphoviridae phage will have a longer tail this means that it has a more gentle approach when it integrates itself into the host cells DNA. This tail allows it to not force it self in but rather inject itself

  5. What obstacles did you run into if any and how would you have approached them in hindsight?

    1. The first time that we ran dna isolation we didn’t have a high quality gel it was only high in quantity. So the DNA isolation had to be run again. If I had more time I would want to take the second DNA gel and go head and use to PCR cluster. I just wish that I had more time

  6. What obstacles did you run into and how would you approach them in hindsight

    1. This was down through an enrichment purfaction titer lysate and DNA isolation prosess .

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