Generally, the host-range breadth describes the types of organisms one is capable of infecting. So in our case, it will allow us to have a greater range of bacteria that our phage is capable of infecting which will allow us to compare it more directly to documented phage and further classify it.
Generally, the host-range breadth describes the types of organisms one is capable of infecting. So in our case, it will allow us to have a greater range of bacteria that our phage is capable of infecting which will allow us to compare it more directly to documented phage and further classify it.
Yes, further characterizing our phage will allow us to know what antibiotic-resistant bacteira our phage is capable of infecting as well as confirm the cluster and identify of our phage.
Hi Julia– great poster! You concluded that you only had one phage because of your EM results, even though you had plaques of varying sizes. How/why do you think one phage could create plaques with such a large variation in diameter?
Thank you! Plaque size usually has to do with the diffusion rate of the phage. So likely, our phage diffuses at different rates when infecting bacteria. It is a bit unusual to have a phage of varying plaque size, so there is not a ton of research on why it occurs.
Yes, it is possible that we still have differing species of phage. While we are fairly confident in our conclusions, in the future we would like to perform further characterization tests to confirm that we turly have one species of phage.
What elements of the further characterization that you mentioned would allow you to better understand the potential applications of this species of bacteriophage in phage therapy?
The best element of further classificaiton to understand our phage’s potential in phage therapy is the host-range breadth. This testing will determine what type of bacteria our phage is capable of infecting which will broaden our knowledge of the applications our of phage.
What will the host range test help you characterize your phage?
Generally, the host-range breadth describes the types of organisms one is capable of infecting. So in our case, it will allow us to have a greater range of bacteria that our phage is capable of infecting which will allow us to compare it more directly to documented phage and further classify it.
How will the host range test help you characterize your phage? What does it specifically tell you?
Generally, the host-range breadth describes the types of organisms one is capable of infecting. So in our case, it will allow us to have a greater range of bacteria that our phage is capable of infecting which will allow us to compare it more directly to documented phage and further classify it.
Will further characterizing your phage allow you to target more specific bacteria?
Yes, further characterizing our phage will allow us to know what antibiotic-resistant bacteira our phage is capable of infecting as well as confirm the cluster and identify of our phage.
Hi Julia– great poster! You concluded that you only had one phage because of your EM results, even though you had plaques of varying sizes. How/why do you think one phage could create plaques with such a large variation in diameter?
Thank you! Plaque size usually has to do with the diffusion rate of the phage. So likely, our phage diffuses at different rates when infecting bacteria. It is a bit unusual to have a phage of varying plaque size, so there is not a ton of research on why it occurs.
Is it possible that the 2 phages could have looked the same on the EM results, but been differing ones?
Yes, it is possible that we still have differing species of phage. While we are fairly confident in our conclusions, in the future we would like to perform further characterization tests to confirm that we turly have one species of phage.
What elements of the further characterization that you mentioned would allow you to better understand the potential applications of this species of bacteriophage in phage therapy?
The best element of further classificaiton to understand our phage’s potential in phage therapy is the host-range breadth. This testing will determine what type of bacteria our phage is capable of infecting which will broaden our knowledge of the applications our of phage.