9 thoughts on “P162 – Howard

  1. Great presentation! Which method of study, out of the electron microscopy, plate creation, and agarose, was your favorite to perform?

    1. My favorite was performing agarose gel experiments not only because its essential for advancing our understanding of the fundamental mechanisms of life and developing new medical treatments but because its cool to apply the electric current to the gel and watch the molecules migrate through the gel-matrix based on their size and charge. Smaller molecules move faster and travel farther through the gel, while larger molecules move more slowly and remain closer to the loading wells.

  2. Your phage has a high DNA content meaning that it is a potential candidate for phage therapy, what would be the titer that would indicate a low DNA presence?

    1. The titer of a phage is not directly related to its DNA content. However, a low titer could indicate a low concentration of phages in the solution, which may or may not be related to the DNA content of the phages.

  3. This was a great presentation! Does the structure of the Lytic Syphoviridae bacteriophage, specifically the long, non-contractile tail, influence its function of being able to lyse a bacterial cell?

    1. Yes, the structure of the long, non-contractile tail in the Lytic Syphoviridae bacteriophage plays a critical role in its ability to infect and lyse bacterial cells. The tail fibers at the distal end of the tail are responsible for recognizing and binding to specific receptor molecules on the surface of the bacterial cell. The tail fibers recognize and bind to receptor molecules on the bacterial cell surface, while the tail sheath contracts to inject the phage genome.

  4. Hey, Great presentation!
    How confident are you that the nanodrop concentration assay was performed correctly? Did you use an average of more readings?

    1. I am confident that I followed the procedure with the supervision of a TA to correctly perform the nanodrop concentration assay. However, I only used the average of two readings one of which was for practice

      1. we were able to conduct 2 restriction digest experiments after that revealed our Phage to be part of the B7 cluster. The B7 phage cluster, also known as the B7-like viruses, has shown potential for phage therapy based on its lytic activity against certain bacterial strains. this enhanced our belief that the results we got in the quality control gel were accurate.

Leave a Reply