A titer assay plate is created by diluting a highly concentrated solution of phage with buffering components to create an agar plate that has between 20-200 plaques. When 20-200 plaques are present you can the calculate the titer of your concentrated phage solution, called HTL, to see how many plaque forming units are present per milliliter.
A titer assay pate is created by using a highly concentrated solution of phage and combining it with buffer components to get an agar plate that has between 20-200 plaques present. Once you have between 20-200 plaques present, you can calculate the number of active phage particles present in your concentrated solution, called HTL.
A titer assay plate is used to help calculate the number of active phage particles present per milliliter of HTL. It is created by combining HTL with buffer components to make an agar plate that contains 20-200 plaques. Once you know the number of plaques and the dilution factor, you can calculate the overall titer.
Thank you very much! Since the quality control gel showed that the DNA was usable for further experimentation, I believe that the smudging in the restriction digest was due to the restriction enzymes themselves. The enzymes must stay on ice at all times to keep them viable, so perhaps our enzymes did not stay cool enough or they didn’t thoroughly mix with the DNA which would also account for the lack of cut DNA.
You said you would redo the restriction digest. Would you do anything differently for this digest? Or why do you think it gave insufficient results in the first place?
Yes, I would like to redo the restriction digest with the same enzymes but changing their incubation time. Ideally, I would allow 72 hours for the restriction enzymes to incubate with the DNA samples in order to let them cut the DNA better. I think the poor results during the first trial were because of the incubation duration as well as temperature.
You said that you would redo your restriction digest. Would you do anything differently this time, and why do you think your results were insufficient this time?
What is a platforming unit?
A plaque forming unit is an active phage particle that is capable of infecting and lysing the host cell.
What is the tidar assay plate?
A titer assay plate is created by diluting a highly concentrated solution of phage with buffering components to create an agar plate that has between 20-200 plaques. When 20-200 plaques are present you can the calculate the titer of your concentrated phage solution, called HTL, to see how many plaque forming units are present per milliliter.
A titer assay pate is created by using a highly concentrated solution of phage and combining it with buffer components to get an agar plate that has between 20-200 plaques present. Once you have between 20-200 plaques present, you can calculate the number of active phage particles present in your concentrated solution, called HTL.
A titer assay plate is used to help calculate the number of active phage particles present per milliliter of HTL. It is created by combining HTL with buffer components to make an agar plate that contains 20-200 plaques. Once you know the number of plaques and the dilution factor, you can calculate the overall titer.
Great job on the presentation! Why do you think the cuts on the restriction digest showed up blurry.
Thank you very much! Since the quality control gel showed that the DNA was usable for further experimentation, I believe that the smudging in the restriction digest was due to the restriction enzymes themselves. The enzymes must stay on ice at all times to keep them viable, so perhaps our enzymes did not stay cool enough or they didn’t thoroughly mix with the DNA which would also account for the lack of cut DNA.
Great job on your presentation! Why do you believe the restriction enzyme came out blurry and made the cuts unclear?
Good job on your presentation! Why do believe the restriction enzyme came out blurry and the cuts became unclear
You said you would redo the restriction digest. Would you do anything differently for this digest? Or why do you think it gave insufficient results in the first place?
Yes, I would like to redo the restriction digest with the same enzymes but changing their incubation time. Ideally, I would allow 72 hours for the restriction enzymes to incubate with the DNA samples in order to let them cut the DNA better. I think the poor results during the first trial were because of the incubation duration as well as temperature.
You said that you would redo your restriction digest. Would you do anything differently this time, and why do you think your results were insufficient this time?