12 thoughts on “P19 – Christiansen

    1. the restriction digestion analysis is looking at and seeing which enzymes cut the DNA of the phage at certain sites. This is helpful to use this in our lab if we were able to continue on the see which cluster our phage may belong to.

  1. Are there any long-term experiments for effectiveness you would like to perform on this phage?

    1. Yes! The long term experiments i would like to perform along with my lab partner is to go back to purification to have isolated our own singular phage, instead of having the containment. This is inhibited our ability to truly see what cluster our phage belonged to since we had two sets of phage DNA.

    1. The benefit of knowing the morphology of your phage is so you can be able to tell if if its siphoviridae or myoviridae phage along with being able to understand if it has the morphology to undergo a lytic or lysogenic life cycle.

    2. The benefit of knowing the morphology of the phage is to know if it is siphoviride or myoviridae and thus to know if the phage will go undergo the lytic or lysogenic lifecyle.

  2. If your phage was not contaminated, how might you proceed with your results? Is the phage only specific to Boulder?

    1. In reality and since phage therapy is so new we don’t know if our phage would be authentic to Boulder it may be found in another state or country for that matter. If our phage was not contaminated we would continue with PCR and try to identify our phage cluster.

    1. I think i would precede with phage therapy, but the changing genome would only be caused by mutations that are introduced to the DNA.

    2. I would want to continue with phage therapy, but the genome can be changed if the DNA is introduced to a mutant.

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