The role of the phage buffer in the mixture is to store the phage in enough liquid substance to be pipetted and used for future experiments without damaging the phage.
Lytic bacteriophage can only undergo the lytic cycle which immediately begins to lyse the bacterial cell, while temperate bacteriophage can undergo either the lytic or the lysogenic cycle, and the lysogenic cycle is when the phage integrates its genome into the host cell’s genome so when the cell replicates the temperate phage genome is replicated with it. At any point in time under certain conditions, the temperate phage genome can activate and lyse the cell.
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What is the role of the buffer in the phage buffer mixture?
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The role of the phage buffer in the mixture is to store the phage in enough liquid substance to be pipetted and used for future experiments without damaging the phage.
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What is the difference between Lytic bacteriophage and temperate bacteriophage?
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Lytic bacteriophage can only undergo the lytic cycle which immediately begins to lyse the bacterial cell, while temperate bacteriophage can undergo either the lytic or the lysogenic cycle, and the lysogenic cycle is when the phage integrates its genome into the host cell’s genome so when the cell replicates the temperate phage genome is replicated with it. At any point in time under certain conditions, the temperate phage genome can activate and lyse the cell.
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