12 thoughts on “P12 – Trehan

  1. Was a reason for the type of DNA ladder you used during these experiments? Especially in figure 4, it seems that your sample results are higher than your highest ladder tier, making it hard to quantify the size. Would you change your ladder size in future experiments?

    1. For figure 4 specifically this was just a quality control, so I didn’t need to know the precise size of my DNA just that it was there and in high enough concentration and quality (shown by the lack of smearing and brightness of the bands) to be used in future experiments. But I think for other experiments such as the PCR I would need to ensure my DNA ladder was the correct size compared to my DNA.

    1. Isolating DNA was the most complicated part of our experiments. It was done through a series of steps using phage lysate. We used DNA resin and isopropanol washes to separate the DNA out and then centrifuged.

  2. Knowing that your phage is likely a member of the A, B, or K clusters, do you have a hypothesis on whether or not which one it is, or is future experimentation necessary to differentiate between the clusters.

    1. I think some of the most important properties would be whether the phage is temperate or lytic, if it can infect bacteria you don’t want it to infect, and its efficacy, among other characteristics. This would ensure it can be effectively used to help stop the bacterial infection.

  3. What are some of the properties that you would look for in a phage that is going to be used for phage therapy?

    1. We can make guesses as to which cluster this phage is apart from its morphology seen in the EM picture, but to get concrete results we need to conduct a PCR test which will confirm which exact cluster this phage is a member of.

    1. Isolating DNA was the most complicated part of our experiments. It was done through a series of steps using phage lysate. We used DNA resin and isopropanol washes to separate the DNA out.

  4. What are some properties that you would look for in a phage designed for use in phage therapy?

    1. I think some of the most important properties would be whether the phage is temperate or lytic, if it can infect bacteria you don’t want it to infect, and its efficacy, among other characteristics. This would ensure it can be effectively used to help stop the bacterial infection.

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