8 thoughts on “P13 – Leidig

  1. What from the quality control gel tells the reader the DNA from Moonpie and Corndog are in fact high quality?

    1. The bright bands and the bright spot assay that was produced on the gel tells the reader the DNA from Moonpie and Corndog are in fact high quality.

  2. If you were to perform your experiment again, what could you have done better to ensure that the gel in Figure 5 was easier to read?

    1. If I was to perform my experiment again, I would test my top agar to make sure that it wasn’t contaminated, and perform at least another round of purification on my phage DNA to ensure that the gel in Figure 5 was easier to read the next time around.

    1. Corndog was classified to be in cluster O based on the results of our restriction digest when we imputed them into phage Enzyme tools and from the PCR gel results. Cluster O means that Corndog is a siphoviridae phage and a lytic phage. This means that due to it being a lytic phage, it can be used in phage therapy.

  3. I really enjoyed your presentation! Are there any antibacterial resistant bacteria that this phage could be used with? (or the phage cocktail)

  4. Thank you so much! Antibacterial resistant bacteria that this phage could be used with are Mycobacterium tuberculosis and Mycobacterium leprae. They can be used because they are close on the phylogentic tree to Mycobacterium smegmatis.

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