12 thoughts on “P23 – Hein

  1. This was a great presentation! Could you explain what A and K clusters are a little more in depth?

    1. Thank you! The A and K clusters refer to ways that we characterize phage genomes. For reference, all phage in the A cluster have a genome with at least 50% similarity, and so do all phage in the K cluster. We believed that our phage were in one of those clusters due to a specific number of restriction digest cuts by the enzymes described.

    1. Mutations in the repressor protein are most likely to affect the life cycles, because the repressor protein represses lytic genes. So, a mutation might repress more lytic genes in a temperate phage and make it more lysogenic, or it might be defective and not be able to repress lytic genes, thus all phage will undergo the lytic cycle.

  2. Great presentation! If you got as far as sequencing the genome, it would be interesting to look at the conserved domains within its genome. Do you know what criteria is used to determine phage clusters?

    1. Thank you! Phage clusters are determined based on phage that have at least 50% genome similarity. For example, all phage in the A cluster have at least 50% genome similarity with one another.

    1. Mutations in the repressor protein are most likely to affect the life cycles, because the repressor protein represses lytic genes. So, a mutation might repress more lytic genes in a temperate phage and make it more lysogenic, or it might be defective and not be able to repress lytic genes, thus all phage will undergo the lytic cycle.

  3. Thank you for sharing your findings. Why did you use six different enzymes for your restriction digest? For example, could you have used 3 or 8 instead?

    1. The six enzymes were the enzymes that we had on hand. In theory, the greater number of enzymes would produce more accurate results, because the different numbers of cuts by each enzyme could have been compared to other numbers of cuts made by those enzymes on different phage genomes. You could use any number, but a greater number will give the best results.

    1. Phage can become lytic from the lysogenic life cycle when they are put under stressful conditions. For example, UV light, high temperatures, and various chemicals might induce phage to become lytic and excise their genome.

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