9 thoughts on “P14 – Munoz

  1. Great job on this presentation. It was very clear and easy to follow. In your conclusions you mention that you ran a PCR test to see if the phage was in an O cluster. I understand that your data was inconclusive, but due to the characteristics present would it be possible to assume its cluster. Also for the future how could you prevent the contamination of your specimen. Thanks, and congrats on the great work.

    1. Thank you! Due to the characteristics me and my lab partner initially did predict our phage would be in the O cluster. Because the PCR test was inconclusive we would proceed to conduct the PCR test again and see if the the control was amplified which it could be because of its very similar characteristics to the O cluster. For the future to prevent contamination I would either isolate my bacteria or I would proceed to conduct many purification processes to hope eliminate the contaminate.

    1. To make sure there was no contamination in the future I would proceed to make sure i use the sterile technique properly and in every step. i would make sure that my lab space is sterile and do my best to avoid contamination with object that haven’t sterilized. if the contaminant still made its way into my results I would conduct multiple purification experiments and hope to remove the containment.

  2. Do you think increasing the amount of times you streaked would allow for a more pure result?

    1. Thank you for the question! I do think that if we increases the amount if times we streaked it would allow for a more pure result. I believe that if we made our phage the most pure it could possibly be we could have gotten rid of all unnecessary contaminates and any DNA that isn’t necessary for phage and phage therapy. Increasing the amount of times we purify it will in the long run give us just the phage but if we continue to run it though our PCR the results wont change because it will continue to contain the same phage DNA.

    2. I think that increasing the amount of times we purified the phage DNA would allow for a purse result but streaking may not. This is because streaking involves performing this process with the same phage DNA and more streaking doesn’t change the DNA in the phage. To make sure we get the most pure result we would perform multiple purification and dilution processes so that a non contaminated phage would appear.

  3. What are the important aspects of your phage being a temperate phage. Are there implications for application or further experiments because it is temperate?

    1. The important aspect of our phage being a temperate phage is its morphology. Our phage appeared to be stable with a mostly clear and cloudy edge. We could see on the plates that it had not completed any lytic cycle because of its halo edge. Because this phage appeared to be temperate it wasn’t ideal for phage therapy. We could wait for this phage to eventually go into the lytic cycle but ideally a lytic phage would be used because of a temperate phages inability to integrate fully into a host cell and its bacteria. For further experiments we could find ways in which a temperate phage could be used to integrate itself into a hist bacteria but because of its characteristics it may prove to be difficult.

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