8 thoughts on “P15 – Carrillo

  1. Greatly articulated presentation, what could explain the possible DNA degradation between the isolation of your phage DNA and running the restriction digest? Had you seen any degradation in your quality control gel?

    1. We weren’t 100% sure what caused the degradation of our DNA but some of the factors that may have contributed to it could’ve been the freezing and thawing of our DNA samples when they were placed into the freezer and then defrosted when we needed to pipette from them. Another cause may have been mistakes in forgetting to place the samples on ice and leaving them at room temperature. Yes we did see minimal degradation in our quality control gel, but we weren’t able to completely confirm quite yet if it was DNA degradation instead of a mistake in loading our samples into the wells of the gel.

  2. What do the different lengths of the phages imply about their function? Do you think a longer or shorter one would have given you different results?

    1. The different lengths of the phage would more so change the family that the phage belong to. For example, since our contaminant phage had a long tail, it belonged to the siphoviridae family, whereas if we had a short tail, it would belong to the myoviridae family.

  3. This was a really great presentation. Which experiment did you do in order to settle that it was not a K cluster but an O cluster?

    1. We mainly discovered that our phage belonged to the O cluster rather than the K cluster from our EM pictures, as only the “corndog” contaminant was present, rather than the K cluster phage we thought was going to appear. We confirmed that our phage was only part of the O cluster from our PCR experiment.

  4. Too bad about the contamination! Do you think your intended phage was looking promising for phage therapy otherwise, or was it totally inconclusive?

    1. It was slightly inconclusive if our intended phage had the ability to be a promising resource for phage therapy. We believe this is because we wouldn’t have been able to sequence our intended genomic DNA, which would have told us the genetic information that our phage carried.

Leave a Reply