8 thoughts on “P26 – Neilly

  1. You have a great way of explaining the topic and the poster content! I think it was a great video, easy to understand and a very cool experiment. I like the confidence you have when she talks, it makes it look even more professional! great work!

  2. I really like your poster and presentation! Do you know how the temperate phage could be converted to be more lytic like you said could help more with phage therapy?

    1. Thank you! To convert the temperate phages into lytic phages we would have to remove the proteins present in temperate phages that allow their viral DNA to be incorporated into the genome instead of lysing the cell. These proteins include integrase and the repressor protein, which both work to maintain the lysogenic cycle and prevent new viral DNA from being made and lysing the cell. This would be effective because lytic phages always lyse the host cell and kill it which would be useful in fighting against bacterial diseases.

  3. great presentation! was does it being part of the A5 family mean? what are some characteristics of this cluster?

    1. Thank you! The A5 cluster is a group of bacteriophages with very similar DNA sequences, including how they will be cut by restriction enzymes because they will have similar cut sites. Characteristics of this cluster include being of the Siphoviridae morphotype, in which phages have long non-contractile tails, and also being temperate. This specificity dictates what types of bacteria they will infect and how they do so. Thus, it can be concluded phages belonging to the A5 cluster will infect bacteria similar to M. smegmatis and will be cut by restriction enzymes in a similar manner.

  4. Nice presentation! Could you explain why you chose the methods you did in order to achieve results? Is it possible for the process you chose to have been done in any order?

    1. Thank you! Following the methods in the order we did was an important process in our experiments to determine the cluster and identification of our phage. For example, a lot of the steps required the confirmation of presence of phages, only working with a singular type of phage, and even having high enough quality of phage genomic DNA to move on to further tests. It was crucial to perform enrichment to amplify any phages present in our sample to further determine presence of said phages in spot tests. Further on, it was essential to confirm we had high enough quantity and quality viral DNA so that we could perform a restriction digest and polymerase chain reaction, which supported our hypothesis that our phage belonged to the A5 cluster. These experiments couldn’t have been done in any order since the specific steps were performed in a manner to best determine what cluster our phage belonged to!

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