Our research aim was to isolate a novel phage that could potentially be used in future phage research. There are a vast number of unstudied phages, and thus phage library needs to be expanded for further research, so our goal was to contribute to this expansion.
Figure 1 shows our bacteriophage plated with M. smeg. The small plaques that you can see are areas where the phage has killed surrounding bacteria. The morphology of these plaques reveals what type of phage, lytic or temperate, the phage is.
Figure 1 shows our isolated phage plated with M. smeg. The cleared regions show where the phage has lysed and killed the surrounding bacteria. The morphology, shape, and size of the plaques help us to determine what type of phage we have.
Great presentation! what were your research aims exactly?
Our research aim was to isolate a novel phage that could potentially be used in future phage research. There are a vast number of unstudied phages, and thus phage library needs to be expanded for further research, so our goal was to contribute to this expansion.
I really enjoyed this presentation! What is going on in figure 1? Make sure to talk about all figures on the poster
Figure 1 shows our bacteriophage plated with M. smeg. The small plaques that you can see are areas where the phage has killed surrounding bacteria. The morphology of these plaques reveals what type of phage, lytic or temperate, the phage is.
Figure 1 shows our isolated phage plated with M. smeg. The cleared regions show where the phage has lysed and killed the surrounding bacteria. The morphology, shape, and size of the plaques help us to determine what type of phage we have.
Did you encounter any unexpected results or issues during your experiments?
The primary unexpected issue that we encountered was our contaminant phage. Another was the degradation of our phage DNA before we ran our final PCR.
Great job! Are there only two life cycles?
Yes, the phage can only undergo the lytic and lysogenic lifecycles.
Yes, the phage can only undergo the lytic and the lysogenic life cycles.