Would you decipher which cluster or family your phage was in using the phase enzyme tool sort of like BLAST where
we use that to see which organisms DNA sequences are most similar?
How would your Phage being a singleton vs a member of group A impact the future tests you would run, are they treated differently given their grouping?
The phage being a singleton would surely complicate future tests, as we would have to guess the most likely cluster it’s in. But if this were the case, we would probably still run the tests the same way, that is, testing for A cluster because those were the closest results.
At one point while conducting the titer assays, we had to redo the experiment 5 times because we kept getting muddy plaques. Eventually, we finally found the source of contamination which was our phage buffer.
The cloudiness of the plaques and the type of phage are correlated because cloudiness indicates that bacteria were not killed in absolution when in contact with the phages, indicate temperate phage.
Would you decipher which cluster or family your phage was in using the phase enzyme tool sort of like BLAST where
we use that to see which organisms DNA sequences are most similar?
Hypothetically, yes, we would use something like BLAST or Phamerator.
How would your Phage being a singleton vs a member of group A impact the future tests you would run, are they treated differently given their grouping?
The phage being a singleton would surely complicate future tests, as we would have to guess the most likely cluster it’s in. But if this were the case, we would probably still run the tests the same way, that is, testing for A cluster because those were the closest results.
Were there any challenges you faced when isolating JaTyrus?
At one point while conducting the titer assays, we had to redo the experiment 5 times because we kept getting muddy plaques. Eventually, we finally found the source of contamination which was our phage buffer.
you used the cloudiness of the plaques to help determine what type of phage you had, how are these two things correlated?
The cloudiness of the plaques and the type of phage are correlated because cloudiness indicates that bacteria were not killed in absolution when in contact with the phages, indicate temperate phage.