Thank you for your question! I believe the next step that would be necessary prior to considering utilizing LilWill as a phage in phage therapy would be to conduct a successful restriction digest and PCR. In doing so, we would be able to identify the cluster/subcluster that LilWill belongs to, and then conduct further testing or sequence the phage DNA to then compare if this phage would interact with a specific bacterium more efficiently, or be utilized in a phage cocktail (which ultimately would require clinical testing prior to implementing LilWill as a phage used in phage therapy).
Wow, your presentation was really well done! Could you explain how a plaque shows you what the morphology of a phage is? Also why is your phage called LilWill? Thank you!
Wow, what a wonderful presentation. COuld you explain what a plaque is and how you can tell a phage’s morphology based on a plagues presention? Also why is your phage called LilWill?
Thank you so much! A plaque is a demonstration that the phage is interacting with the bacteria, as the small clearings that you see are caused by the phage lysing the bacterial cells. The plaques are clear and lytic when this clean, circular, completely clear clearing in the agar is shown, because the phage is completely lysing all of the cells in that area. If the plaque had a halo, and was therefore lysogenic, it’s undergoing both lifecycles, the lytic lifecycle being evidenced by the slight clearing you still see, and the blurry halo showing how not all of the cells are being fully lysed while the phage is integrating into the host cell’s genome. We named our phage LilWill because the soil sample used to isolate the phage was found in Williams Village, and our plaques were consistently “little.”
Thank you for your question! The lack of a halo and clear, circular shape of the plaques proves that the phage is lytic because it means that all of the bacteria where the plaque is has been completely lysed, and causing that clean clearing on the plate.
Outstanding poster and presentation! Do you believe one type of phage, lytic or lysogenic, would be better for phage therapy or would they both be beneficial to the host?
what experiment from your future direction do you think would be the most important next step when verifying your phages potential in phage therapy?
Thank you for your question! I believe the next step that would be necessary prior to considering utilizing LilWill as a phage in phage therapy would be to conduct a successful restriction digest and PCR. In doing so, we would be able to identify the cluster/subcluster that LilWill belongs to, and then conduct further testing or sequence the phage DNA to then compare if this phage would interact with a specific bacterium more efficiently, or be utilized in a phage cocktail (which ultimately would require clinical testing prior to implementing LilWill as a phage used in phage therapy).
Wow, your presentation was really well done! Could you explain how a plaque shows you what the morphology of a phage is? Also why is your phage called LilWill? Thank you!
Wow, what a wonderful presentation. COuld you explain what a plaque is and how you can tell a phage’s morphology based on a plagues presention? Also why is your phage called LilWill?
Thank you so much! A plaque is a demonstration that the phage is interacting with the bacteria, as the small clearings that you see are caused by the phage lysing the bacterial cells. The plaques are clear and lytic when this clean, circular, completely clear clearing in the agar is shown, because the phage is completely lysing all of the cells in that area. If the plaque had a halo, and was therefore lysogenic, it’s undergoing both lifecycles, the lytic lifecycle being evidenced by the slight clearing you still see, and the blurry halo showing how not all of the cells are being fully lysed while the phage is integrating into the host cell’s genome. We named our phage LilWill because the soil sample used to isolate the phage was found in Williams Village, and our plaques were consistently “little.”
Why is the lack of a halo on one of your plaques proof that the phage is lytic?
Thank you for your question! The lack of a halo and clear, circular shape of the plaques proves that the phage is lytic because it means that all of the bacteria where the plaque is has been completely lysed, and causing that clean clearing on the plate.
Outstanding poster and presentation! Do you believe one type of phage, lytic or lysogenic, would be better for phage therapy or would they both be beneficial to the host?