11 thoughts on “P31 – Johnson

    1. Hello, in reference to your question, the methods followed a standard approach to be able to isolate a phage from a collected dirt sample. Starting with the dirt sample which was then followed by the enrichment process that when plaques were shown was taken through many spot tests and dilutions to obtain one kind of plaque. Further dilutions were done to determine web pattern plates and obtain an eventual high titer lysate. The lysate was then used to obtain the EM image and this is all the methods my partner and I were able to do with successful results.

  1. How did you identify the phage as lytic? Can this affect its process as a potential phage therapy?

    1. Hello, I identified the phage LilWill to be lytic after the consistency of the all the plates (between the spot tests, dilutions, and web patterns) showed to have small clear circular plaques on the plates with no halos. This clearness is an indicator that the phage is lytic as it follows the lytic life cycle by lysing the bacteria completely shown by the clear plaque. To speak to your second question, lytic phage are predominantly used in phage therapy due to them completely lysing the bacteria each time. This efficiency and effectiveness of lytic phage make them a more suitable candidate to be used in phage therapy then temperate phage which undergo the lysogenic life cycle making it more difficult to completely lyse the bacteria.

  2. Hi great job on your presentation and poster! Why is it important that the phage is lytic? Thanks, and great job again!

    1. Hello, in reference to your question, I am unsure what you completely mean as there is not an importance if phage LilWill is temperate or lytic. However, with phage LilWill being lytic is makes it a more suitable contender for phage therapy as lytic phage only follow the lytic lifecycle meaning they completely lyse the bacteria. Temperate phage, on the other hand, can undergo both lytic and lysogenic life cycle, which would mean editing should be done to the temperate phage to make it a suitable contender for phage therapy. Thus, with LilWill being lytic it is a better contender for phage therapy as little editing would need to be done on it just further experimentation to fully see how it interacts with other bacterias.

  3. Nice job! How relevant is morphology to the phage’s ability to lyse a cell?

    1. Hello, based on this lab, I was not able to draw a line to see the actual relevance of LilWill’s siphovirade morphology to it’s ability to lyse a bacteria cell. There are assumptions based on the long non contractile tail which makes it difficult or more effective in binding to the receptors on the bacteria cell which would then affect the overall ability of the phage to lyse the cell. However, I do not know for sure that relevance and all I can offer are assumptions based on what is known about phage morphology and other research studies out there.

    2. Hello, based on what I was able to do in this lab, I was unable to identify a clear relevance between LilWill’s siphovirdae morphology and its overall ability to lyse a bacteria cell. Overall, it is known that the siphovirdae morphology has the long non-contractile tail which could affect how well the phage is able to bind to the receptor proteins on the bacteria cells, but from what I was able to determine, I could not find a direct correlation between the two.

  4. Nice job! How relevant is the phage’s morphology to its ability to lyse a cell?

  5. Hi there! How are phages applied as treatment? Are they taken orally or as injections?

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