15 thoughts on “P19 – Hargrove

  1. Was your research able to identify the identity of the two isolated phages, or did contamination prevent that?

    1. Since there was contamination, it prevented to get a clear idea what type of cluster the phages were. As for the contaminant’s cluster, that information was already given to us as it was being spread through out the lab and other groups had issues too.

  2. Hi Genevieve! This was a really cool project. Bummer that you were unable to isolate a single phage, but still really cool that you were able to notice that (also love that it is called/you called it a corndog because that made it super clear what you talking about). I have two questions:

    1) If you were able to isolate one phage, what would have been the next step and the potential future outcome of having it? Like is it just to keep on hand in a sort of phage library to use to test/document later on, or is there some other reason?

    2) Is corndog the actual name? Because if so that’s awesome, if not then well done keeping my attention!

    Tell Dr. Guild hello from the old postbacc with long hair that haunts Foolish Craig’s.

    1. So to answer your first question, the next step would have been to archive it so that it would be able to have potential use for phage therapy and would be sent to a different lab to do multiple tests on the phage. As well the name of the contaminant phage is Corndog!

  3. The poster and presentation were great!! What were some of the strategies that you tried in order to try and isolate a single phage? Did you try to isolate specifically the non-O cluster phage, and how would you go about trying to pick out a phage to isolate and archive?

    1. So the strange part of our experiment is that everything was going accordingly and we should have found the contaminant within the early processes of the experiment; however, it came way later than it should have so we did not have enough time to isolate a singular phage. But the process of isolating a singular phage is through multiple processes of Purification which involves a lot of serial dilutions and flooding a web pattern plate to concentrate it. Answering your second question, we did try to specifically isolate the non-O cluster phage as we were seeing more plaques of that species than the contaminant. Normally picking out a phage to isolate is we go through the same processes of purification and web pattern plates to get that one type, so there must have been something wrong with an external tool that kept causing the contaminant (we think it might have been the buffer we were using). Then once there is a isolate phage, we would do a restriction digest then PCR to conclude our results and then archive for later purposes such as phage therapy.

  4. I was also wondering how any contamination would affect identify the isolated phages. Also what type of contamination would there be?

    1. So the goal of our research was to only get one type of isolated phage, with the contaminant being there that causes two different types and would make our results weird as we are testing more than one subject. I think I’m answering your second question right but we thought maybe that the contamination was within the phage buffer we used, but we are not really sure how it was contaminated.

  5. Thank you for sharing! Another question about contamination – how do you think it occurred? How could the contamination have been prevented?

    1. So the funny part is, we are not entirely sure how it occurred. It was very strange as we were further down into our experiment and would have known if we had a contaminant within the earlier stages, but our results of a contaminant came out of no where. We think it might be our phage buffer, but not entirely sure. Preventing contamination normally within a lab and what we did, was to use a Bunsen Burner to keep things sterile. Another thing too is by constantly changing the pipette tips with new sterile ones.

  6. What are all the different types of clusters you used for this experiment and what was their purpose?

    1. So clusters that we would have used would have been ones during the PCR experiment as we were trying to determine what cluster our phage were, but since we did not get to that we didn’t get a chance to do that. Basically a ‘cluster’ is a group of similar phages and this can help classify the phage and its future use.

  7. Thanks for your work! But why did not isolating a singular phage prevent you from finishing the experiment? Great job!

    1. A large part of it was we were trying to get that singular phage isolated but just ran out of time!

  8. Thanks for sharing! Why did the contamination prevent you from finishing the project?

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