6 thoughts on “P36 – Ferguson

  1. Great presentation! How would the dilution of the lysate, hopefully that’s the right name, effect the other results? Would it change for every phage? Would an increase in lysate dilution work just as well?

    1. Once a High Titer lysate was made through purification of phage DNA and flooding of a web pattern plate, 8 1:10 dilutions were made to plate on a spot test. The lysate only produced clearings in the 10^-1 through 10^-7 spots on the plate which is how we determined that we should dilute it to 10^-7 to produce a 20-200 plaque plate to calculate the titer. The dilution did effect our results because, as we expected, the 10^-7 dilution produced our 20-200 plaque plate. Yes, the dilution factor does change for different phage due to the concentration of phage DNA in the lysate. Finally, an increase in lysate dilution would not work because as I said before, our phage only produced clearings to 10^-7. The 10^-8 dilution removed the presence of observable phage DNA in the lysate.

    1. From the earliest observable results of this experiment, the only thing that I was expecting was that our phage was lytic. This turned out to be true, however, after receiving the EM images of our phage, we saw that we had a “corndog phage”. Corndog phage is thought to be a contaminant in the lab and cannot be archived which was an unfortunate and unexpected surprise.

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