Lytic phages are preferred for phage therapy because they undergo the lytic cycle where once integrated into the host genome it immediately kills the bacterial cell opposed to temperate phage which could undergo the lysogenic cycle where upon integration it does not lyse the bacterial cell until certain environmental stressors inflict it to. This means that in terms of phage therapy lytic phage are more reliable for immediately killing bacterial cells opposed to temperate phage.
Thank you, your presentation as well. It is important to know that the phage was temperate because this classification will automatically make it less desirable for phage therapy because it could enter the lysogenic cycle (and not lyse the bacterial cell). It is also important to know for future research regarding genome sequencing and gene editing, for example if it is temperate we can look for and possibly remove the repressor protein to allow it to go into the lytic cycle upon integrating its genome into the bacterial cell.
A temperate phage is a phage that can either enter two cycles upon entering the bacterial cell, (rather than a lytic phage who only enters one, the lytic cycle) either the lytic or lysogenic cycle. It is significant because if a temperate phage enters the lysogenic cycle it is less desirable for phage therapy since it does not kill the bacterial cell until certain environmental conditions prompt it to. A lytic cycle is categorized by lysing (killing) of the bacterial cell after integration of the phage DNA. A lysogenic cycle is characterized by integration of viral DNA into the bacterial host where it lays dormant until prompted by environmental conditions.
How did analyzing restriction digestion cuts help you determine your results? What about that procedure tells you that they are similar to B7 cluster phages?
Restriction digests allow you to visualize DNA sequence segments based on how restriction enzymes cut the fragments . We can compare cuts from our phage DNA sequence to other phage restriction digest patterns via phage enzyme tools which give us results of similar cuts from various clusters. We can cross compare our cuts to our top cluster results via the enzyme tool and this allows us to hypothesize that our phage belongs to the cluster with the highest genome similarity via the DNA fragment cut patterns.
Excellent presentation Violet!! You were fantastic all throughout the semester and I loved interacting with you and your group! So proud to see how far you got with your phage!
curesymposium.org 
Do you know why lytic phages are preferred in phage therapy?
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Lytic phages are preferred for phage therapy because they undergo the lytic cycle where once integrated into the host genome it immediately kills the bacterial cell opposed to temperate phage which could undergo the lysogenic cycle where upon integration it does not lyse the bacterial cell until certain environmental stressors inflict it to. This means that in terms of phage therapy lytic phage are more reliable for immediately killing bacterial cells opposed to temperate phage.
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Very well articulated! My question was why was it important that the phage was temperate?
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Thank you, your presentation as well. It is important to know that the phage was temperate because this classification will automatically make it less desirable for phage therapy because it could enter the lysogenic cycle (and not lyse the bacterial cell). It is also important to know for future research regarding genome sequencing and gene editing, for example if it is temperate we can look for and possibly remove the repressor protein to allow it to go into the lytic cycle upon integrating its genome into the bacterial cell.
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what is a temperate phage and what makes that significant?
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A temperate phage is a phage that can either enter two cycles upon entering the bacterial cell, (rather than a lytic phage who only enters one, the lytic cycle) either the lytic or lysogenic cycle. It is significant because if a temperate phage enters the lysogenic cycle it is less desirable for phage therapy since it does not kill the bacterial cell until certain environmental conditions prompt it to. A lytic cycle is categorized by lysing (killing) of the bacterial cell after integration of the phage DNA. A lysogenic cycle is characterized by integration of viral DNA into the bacterial host where it lays dormant until prompted by environmental conditions.
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How did analyzing restriction digestion cuts help you determine your results? What about that procedure tells you that they are similar to B7 cluster phages?
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Restriction digests allow you to visualize DNA sequence segments based on how restriction enzymes cut the fragments . We can compare cuts from our phage DNA sequence to other phage restriction digest patterns via phage enzyme tools which give us results of similar cuts from various clusters. We can cross compare our cuts to our top cluster results via the enzyme tool and this allows us to hypothesize that our phage belongs to the cluster with the highest genome similarity via the DNA fragment cut patterns.
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Excellent presentation Violet!! You were fantastic all throughout the semester and I loved interacting with you and your group! So proud to see how far you got with your phage!
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