8 thoughts on “P22 – Harlan

  1. Thanks so much for your presentation! Can you explain what the significance of a bacteriophage being in an O-cluster would be in terms of clinical applications/

    1. Knowing the cluster would allow us to group the phage with other phages in the cluster. Grouping the phage would allow us to have a better understanding of the host range. Having that information would allow someone looking for a candidate phage to know what our phage could possibly be used to infect.

  2. Hey! Great work on your presentation! I’m curious about what the implications of a phage in O-cluster are? I’m not sure I fully understand why it is important if a phage is in O-cluster versus a K-cluster when it comes to antibiotics resistance. Please let me know when you get a moment 🙂 Have a nice summer!

    1. It is less about antibiotic resistance but about what bacteria the phage can infect. If we know the cluster we can connect the phage to that cluster and the known host ranges associated with the cluster. A K cluster phage may be able to infect different bacteria than an O cluster phage. For someone looking for a candidate phage to treat an infection, they would want to know the host range and thus cluster. Thank you, Have a nice summer.

  3. Hi! Great work on your bacteriophage and presentation! I was wondering since your isolated bacteriophage is lytic, was it more applicable in treatment of antibiotic resistant bacteria? What is the significance of your bacteriophage being lytic?

    1. When treating a bacterial infection it makes more sense to use a lytic phage because it will lyse the cell wall and kill the pathogen cell. A temperate phage would inject its DNA and not kill all of the cells. Since the goal is to kill all of the bacteria that are causing the infection it would definitely be more useful to use lytic phage. Thank you.

  4. Thank you for the presentation. How did you determine it was a siphoviridae phage simply by knowing that the tail was longer than the head?

    1. That statement I made was not entirely correct, unfortunately. It is a characteristic that they have long heads and longtails. I generalized it as the ratio between them is important but that was not correct. You would identify it as a siphovierde by understanding the head and tail are very long and the tail is noncontractile.

Leave a Reply