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13 thoughts on “P45 – Elkinbard”
What is the difference between the lytic and lysogenic life cycle of the phage?
During the lytic life cycle the phage lyses the cell but during the lysogenic life cycle the phage just incorporates its genome into the bacterial genome and thats how it survives
Just wondering how you calculated the high titer lysate, and what does that number mean?
The high titer lysate is the number of plaque forming units of the lysate. I calculated it using this formula:
Titer (pfu/mL) = (pfu (number of plaques)/#µL used for the infection) X (1000µL/1mL) X (dilution factor)
What would you have done differently if you could repeat these experiments in the future?
How would killing these things off help the aqua culture? Would these phages potentially harm the aquaculture at all?
Yes, it would benefit aquacultures by reducing the bacterial population present that is killing them off. For now, It is not believed that phages would harm aquacultures because they only target harmful bacteria
What would you have done differently if you could perform these experiments again?
How would killing off these things help the aqua culture? Could the phages potentially harm the aqua culture?
what would you have done differently if you could do these experiments agains?
Honestly I wouldn’t do anything differently because it lead to the results I was hoping for. I got the opportunity to isolate a mycobacteriophage
Did you encounter any unexpected obstacles during your experiment? If so, how did you combat them and, if able, what would you do in future experiments to avoid these obstacles?
No, I did not encounter any unexpected obstacles. The procedures were very direct and so were the outcomes