We would most likely need direction from our instructors to determine which array of enzymes to use next. My guess would be enzymes that are similar to the ones that showed cuts on our restriction digest results.
After obtaining our phage and enriching it, a spot test was performed. Plaques from the spot test were taken and used to in 4 1:10 serial dilutions (purification). After this, the following plaques shown in figure two were displayed.
No. There was an enzyme (HindIII) that showed potential relevance, however our instructor told us to not pay attention to it since its results couldn’t be confidently determined.
The halo ring results in figure 2 were a major step in our research. The halo rings are part of the plaques morphology and mean that our phage is temperate. To further clarify, the halo rings are areas where bacteria have been infected by our phage, and have phage DNA integrated into their own, and aren’t being killed instantly by other phages.
Since our phage was determined to be temperate, those cloudy halo rings around the plaques are areas where bacteria have been infected with our phage, but aren’t being killed since the phage has integrated its DNA into the bacteria’s own DNA, preventing other phage was killing it.
Being able to group phages into a data base (like our phage being in the O cluster group) could allow future medical use to easily obtain a phages that are similar to one another and attack a particular strain of bacteria.
Being able to group phage into a data base (like our phage being part of O cluster group) could allow medical use to utilize this data base to easily obtain a phage/s that they need to use to treat a specific strain of bacteria.
My best guess is that our phage was just a diluted/purified data of the contamination. We didn’t know if our phage was the contamination phage until after the EM images.
Can you explain a little more on what the experiment was that gave you the results in figure 2? That part was a little unclear.
For your second future direction, how would you select enzymes to perform restriction digests on? What would make an enzyme a good candidate?
We would most likely need direction from our instructors to determine which array of enzymes to use next. My guess would be enzymes that are similar to the ones that showed cuts on our restriction digest results.
After obtaining our phage and enriching it, a spot test was performed. Plaques from the spot test were taken and used to in 4 1:10 serial dilutions (purification). After this, the following plaques shown in figure two were displayed.
I accidentally answered your question under Gabby McBane’s question. Sorry
I accidentally answered your question on Gabby McBane’s feed. Sorry
What were some limitations of your research?
Some limitations was definitely time and contaminations that prevented further research to be done.
Were any of results from the other restriction enzymes you used relevant in determining that this phage was from the O-cluster?
No. There was an enzyme (HindIII) that showed potential relevance, however our instructor told us to not pay attention to it since its results couldn’t be confidently determined.
Great presentation! Are the halo rings that formed in figure 2 apart of the plague morphology and do the mean/do anything specific for the plaques?
The halo ring results in figure 2 were a major step in our research. The halo rings are part of the plaques morphology and mean that our phage is temperate. To further clarify, the halo rings are areas where bacteria have been infected by our phage, and have phage DNA integrated into their own, and aren’t being killed instantly by other phages.
what are the Halo rings?
Since our phage was determined to be temperate, those cloudy halo rings around the plaques are areas where bacteria have been infected with our phage, but aren’t being killed since the phage has integrated its DNA into the bacteria’s own DNA, preventing other phage was killing it.
How does your conclusion relate to the medicinal uses of phage?
Being able to group phages into a data base (like our phage being in the O cluster group) could allow future medical use to easily obtain a phages that are similar to one another and attack a particular strain of bacteria.
Being able to group phage into a data base (like our phage being part of O cluster group) could allow medical use to utilize this data base to easily obtain a phage/s that they need to use to treat a specific strain of bacteria.
Did you end up researching on Ballie after its initial contamination on your experiment or was this the phage your group isolated since the beginning?
My best guess is that our phage was just a diluted/purified data of the contamination. We didn’t know if our phage was the contamination phage until after the EM images.