After determining what type of phage yours was, what might this tell you about its properties? Why did you decide to complete a serial dilution as your method for determining the phage to be lytic, rather why and how did you chose the methods you used?
M. Smegmatis serves as a very safe as well as similar bacteria to that of bacteria in humans so it allows scientists to test phages with M. Smegmatis as a great testing agent with minimal risk.
PCR allows for the analysis of genes in the phage that can then be cross referenced with previously sequenced phages in order to look for similarities to determine the cluster.
Plaques are spots formed after phage have successfully been able to replicate and kill a portion of nearby host bacteria. They form either clear or cloudy plaques which lets us determine if the phage is lytic or temperate.
We named our phage after the sea lion in Finding Dory because we had so many issues trying to get our phage through some of the experiments we conducted.
Why was M. Smegmatis the model organism in this experiment?
M. Smegmatis is a very safe as well as similar bacteria to that of bacteria in humans so it serves as a great testing agent with minimal risk.
After determining what type of phage yours was, what might this tell you about its properties? Why did you decide to complete a serial dilution as your method for determining the phage to be lytic, rather why and how did you chose the methods you used?
A lytic Siphorviridae phage shows key characteristics of being a highly effective target agent than that of other phages such as temperate.
Excellent presentation!
It seems like M. Smegmatis was the ideal model organism across all phage experiments. Why is this?
Also how do the results of a PCR help determine the phage cluster?
M. Smegmatis serves as a very safe as well as similar bacteria to that of bacteria in humans so it allows scientists to test phages with M. Smegmatis as a great testing agent with minimal risk.
PCR allows for the analysis of genes in the phage that can then be cross referenced with previously sequenced phages in order to look for similarities to determine the cluster.
Excellent presentation!
M. Smegmatis seems to be the ideal organism for testing but why is this?
Also how do the results of a PCR allow you to determine the phage cluster?
Hi! What are plaques and why are they necessary for this research? Why is your organism named Jerald?
Plaques are spots formed after phage have successfully been able to replicate and kill a portion of nearby host bacteria. They form either clear or cloudy plaques which lets us determine if the phage is lytic or temperate.
We named our phage after the sea lion in Finding Dory because we had so many issues trying to get our phage through some of the experiments we conducted.