Because these experiments served to answer a question of existence (is there phage present in the soil sample we collected/what kind of phage is present), I don’t think a hypothesis was necessarily applicable, nor did we make one before beginning experiments.
As you mentioned, there wasn’t enough time to continue with your experiment. If you did have the extra time and resources, what would be your next step?
We would have liked to continue several more rounds of purification so Fudge could be isolated and further characterized through DNA isolation and restriction digest, which would give us more insight into the cluster that Fudge belongs to.
Because our phage is temperate, it would be difficult for this phage to be directly used in phage therapies without genome modification. However, the mechanism of genome insertion and incorporation into the host is still very interesting and has potential for further study, perhaps in making beneficial bacteria resistant to infection.
I am sorry and forgive me if I missed it but, What was your hypothesis and did your result support it ?
Because these experiments served to answer a question of existence (is there phage present in the soil sample we collected/what kind of phage is present), I don’t think a hypothesis was necessarily applicable, nor did we make one before beginning experiments.
As you mentioned, there wasn’t enough time to continue with your experiment. If you did have the extra time and resources, what would be your next step?
We would have liked to continue several more rounds of purification so Fudge could be isolated and further characterized through DNA isolation and restriction digest, which would give us more insight into the cluster that Fudge belongs to.
I thought everything was thoroughly explained, but is there any reason for naming the phage “Fudge”?
Nope. Just sounded cute.
How might your results contribute to our understanding and development of phage therapies in the future?
Because our phage is temperate, it would be difficult for this phage to be directly used in phage therapies without genome modification. However, the mechanism of genome insertion and incorporation into the host is still very interesting and has potential for further study, perhaps in making beneficial bacteria resistant to infection.