Other labs ran PCR that confirmed the cluster and we were told it was the same phage by the professors and TA. Unfortunately beside EM we were not able to perform any other experiments that were successful.
This phage contaminated our phage and several others in both of the labs. We believe this could be due to a contamination in the phage buffer when flooding or it could be airborne.
Did the Corndog contamination affect your Fudge phage at all?
While it did not effect the phage itself, it did mean that we could not archive the phage.
What were the methods used to test this aside from performing electron microscopy?
Other labs ran PCR that confirmed the cluster and we were told it was the same phage by the professors and TA. Unfortunately beside EM we were not able to perform any other experiments that were successful.
What is the O cluster?
An O cluster is part of the Siphoviridae family that have a different and unique morphology that differs most phage
I really liked your presentation. Why did you end up having 2 phages and did you end up purifying them from each-other on separate assays?
This phage contaminated our phage and several others in both of the labs. We believe this could be due to a contamination in the phage buffer when flooding or it could be airborne.