8 thoughts on “P34 – Calderon

  1. How did you perform the Restriction Digest? What was the actual process taken to get the results shown in Figure 2?

    1. The restriction digest was run on an agarose gel through the gel electrophoresis process. By loading my phages DNA + a different enzyme to each lane in the gel, I could test to see what enzyme could cut my phages DNA.

    1. I believe there are 30 distinct types of phage clusters or “families.” However, each of those 30 clusters have many many other different sub-clusters. These sub clusters categorize phage that have the closest homology in genes, plaque morphology, and physical appearance.

    1. Characteristics such as plaque morphology, phage morphology, genome size, and genes can factor into the distinction of O cluster phage from other phage. One clear factor to distinguish O-cluster phage however is their long corn dog head.

    1. Finding a lytic phage has a separate purpose than it being O-cluster. A lytic phage must be present for purposes such as making completely clear plaques on agar plates, or when in use for phage therapy. You can think of a lytic phage as an active phage that doesn’t go to sleep. If a patient is treated with phages, you would obviously want the phages to be actively targeting bacterial cells. Now, a phage that is lytic or temperate can place that phage into certain clusters. For the O-cluster, most phage are characterized as being lytic.

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