The lytic cycle is the “default” setting for phage – in order for them to do the lysogenic life cycle, they need to use protein repressors to suppress the lytic genes’ expression. So phages that undergo the lytic cycle would not have the protein repressors stop those genes from being expressed.
We believe that it most likely belongs to the “N” cluster, as the plaque and EM data for this cluster is very similar to ours. However, this is only a prediction and would need to be confirmed with the PCR cluster analysis.
We believe that the isolation of our phage will contribute to the body of knowledge that we have about phage. This means that our phage’s data can be compared to similar and different phages so that we can understand more about phage biology and genomics.
However, because of its temperate lifecycle, and very small plaques, our phage could not be used for phage therapy or for applications where plaques are needed as a visual result.
Why do the phages undergo the lytic cycle instead of lysogenic?
The lytic cycle is the “default” setting for phage – in order for them to do the lysogenic life cycle, they need to use protein repressors to suppress the lytic genes’ expression. So phages that undergo the lytic cycle would not have the protein repressors stop those genes from being expressed.
Do you have a prediction about which cluster your phage might belong to?
We believe that it most likely belongs to the “N” cluster, as the plaque and EM data for this cluster is very similar to ours. However, this is only a prediction and would need to be confirmed with the PCR cluster analysis.
We believe it most likely belongs to the N cluster, based off of plaque and EM data. This would need to be confirmed with a PCR cluster analysis.
If AllyWally does not belong to the K1 cluster, what cluster do you believe it belongs to?
We believe it most likely belongs to the N cluster, based off of plaque and EM data. This would need to be confirmed with a PCR cluster analysis.
Since AllyWally cannot be used for phage therapy, what do you expect it to be useful for?
We believe that the isolation of our phage will contribute to the body of knowledge that we have about phage. This means that our phage’s data can be compared to similar and different phages so that we can understand more about phage biology and genomics.
However, because of its temperate lifecycle, and very small plaques, our phage could not be used for phage therapy or for applications where plaques are needed as a visual result.