9 thoughts on “P37 – Carroll

  1. In your presentation, you mention that you knew that your phage belonged to the K4 subcluster, does this mean that it is most effective against K4 bacteria?

    1. No, this does not mean that Mootaw is most effective against K4 bacteria. It simply means that it is most similar to phage in the K cluster specifically K4 phages. This means that the phage in this category share many of the same genes are most likely applicable to the same types of bacteria as each other.

  2. How, if at all, would your results have differed if Mootaw was discovered to be a lysogenic bacteriophage?

  3. How, if at all, would your results have differed if Mootaw was found to be a lysogenic bacteriophage?

    1. If Mootaw was found to be a lysogenic bacteriophage, genetic modification would have to be done for Mootaw to be used in a phage cocktail for phage therapy. This is because lytic bacteriophage are ideal for phage therapy because they kill all of the bacteria present.

  4. In your presentation you mentioned the cycles that a phage can go through can that change whether or not it’s effective on a certain type of bacteria since you are targeting K4 bacteria?

    1. All phage that were isolated in this course were able to infect mycobacterium smegmatis. This means that it would not affect the phages ability to affect the bacteria whether it is lytic or lysogenic.

    1. Thank you!! Towards the beginning of our research we encountered issues with isolating one species of phage. We had to do multiple rounds of dilutions in order to isolate one type of phage. This caused some timing set backs. After overcoming this hurdle, we also ran into the issue of contamination of another phage species.

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