12 thoughts on “P68 – Millizo Kroll

  1. Could you explain a little bit more about what it means that your phage follows patterns of the F1 cluster? Thank you!

    1. Phage are put into different clusters based on their specific characteristics. One such characteristic is the specific cutting from restriction enzymes. Our phage was cut by the same restriction enzymes as phage in the F1 cluster, which implies that our phage is also in that cluster (but still needs to be confirmed).

  2. Based of figure 3, how are you determining which cluster your phage belongs to? What are you looking for on that gel to determine this? Also, what PCRs would you run to confirm your cluster?

    1. We used an online tool, the PET simulator, where you input which restriction enzymes your phage was cut by and it compares it to other phages and phages in specific clusters. We are looking for bright bands that indicate the phage was cut by the specific restriction enzyme.

  3. Based on your cluster hypothesis of F1, is there certain limitation your phage may have when it comes to the mentioned future experiments with in vivo testing? Or any idea of how it being F1 could help?

    1. Hypothesizing that it is in F1 does not imply that there are certain limitations. It also doesn’t necessarily help, it just tells us what specific characteristics it may also have.

    2. Characterizing it as F1 does not pose any limitations or strengths, but simply further gives specific characteristics it may have that could be helpful to determine which specific bacteria it would fight off successfully.

    3. Characterizing the phage as F1 does not impact the effectiveness, it just further characterizes it which could be helpful for determining which bacteria it would be good to fight against in the future.

  4. I really enjoyed your presentation; I found it very concise and easy to understand. I just have one clarifying question, how would you determine which cluster your phage belongs to? What would knowing this information do to benefit your research?

    1. We determined which cluster our phage belonged to by using the Phage Enzyme Tools website. This helps further characterize the phage which can be helpful to see how it would be used against specific bacteria in the future.

  5. Great presentation, very easy to follow and understand. I just wondered about how you read your plates and how you were able to determine which phage came from which cluster.

    1. We counted the plaques in each plate and noted whether they were clear or cloudy. We also determined which cluster it belonged to using the Phage Enzyme Tools website, which allowed us to input our results and compared it to phage in other clusters.

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