12 thoughts on “P37 – Robinson

  1. Do you have any ideas as to how Mootaw was contaminated with an O cluster phage when doing the electron microscopy?

    1. My partner and I speculate two different ways that our phage may have been contaminated. Firstly it is possible that our entire lysate sample was contaminated with O cluster phage the entire time, and when EM is performed an extremely small amount of lysate is used to image. It is entirely possible that the person who imaged our phage managed to only pick up O cluster phage in the sample that was imaged. Alternatively, we could conclude that the machinery used in EM was contaminated altogether, because mostly every other group in the class also found contaminated phage that belonged to the O-cluster. Due to this strange amount, a mass-contamination is most likely in this case.

    1. As explained in Figure 1, a lytic phage kills (lyses) the host cell almost immediately whereas a temperate phage lies dormant in the host cell and then lyses it whenever it wants. The fact that Mootaw was a lytic phage was very important to our findings because this means that it is a better candidate for phage therapy due to its ability to kill the harmful bacteria immediately.

    1. It is important to determine Mootaw’s host range because it will allow us and researchers to compare it to existing similar phages and this makes it easier to find for making phage cocktails in therapy.

    1. It is important to determine Mootaw’s host range because it will allow us and researchers to compare it to existing similar phages and this makes it easier to find for making phage cocktails in therapy.

  2. Did you not see any bacteriophage that looked like K4? If not, why do you think you only saw O?

    1. No, we did not see any phage that looked like they belonged to our predicted phage cluster K4.
      My partner and I speculate two different ways that our phage may have been contaminated. Firstly it is possible that our entire lysate sample was contaminated with O cluster phage the entire time, and when EM is performed an extremely small amount of lysate is used to image. It is entirely possible that the person who imaged our phage managed to only pick up O cluster phage in the sample that was imaged. Alternatively, we could conclude that the machinery used in EM was contaminated altogether, because mostly every other group in the class also found contaminated phage that belonged to the O-cluster. Due to this strange amount, a mass-contamination is most likely in this case.

  3. You don’t really explain in your presentation how the permissive temperature experiment will shape your results, what do you hope to find from this?

Leave a Reply