Plaques are areas where phage have cleared out the bacteria by reproducing inside of them and then bursting out of the bacteria essentially killing the bacterial cell. If there is no production of plaques there must have been contamination or you had no phages to begin with.
What does HTL mean?
HTL stands for the high titer lysate which is the concentrate of phage in the lysate
What make up plaques and what would be the reason if there is no production of plaques?
Plaques are areas where phage have cleared out the bacteria by reproducing inside of them and then bursting out of the bacteria essentially killing the bacterial cell. If there is no production of plaques there must have been contamination or you had no phages to begin with.
Why were you not able to run the restriction PCR?