8 thoughts on “P38 – Tuite

  1. Did groups with similar phage observe similar results? Also was the size of your phage big or small?

    1. anyone who was able to isolate a corndog phage had the conclusion that it was most likely isolated via contamination, they either went on to be able to determine whether or not it was o cluster via PCR, results of which I’m not completely sure. the head size of our phage was relatively larger than most mioveridae and siphiovaridae since it was so long.

    1. we typically determine the cluster of the phage to be able to determine the genome of said phage as well as to compare it to other o cluster phages!

  2. How do you think in the future you would be able to determine wether or not the results were derived from the actual soil sample or as you said contamination? Do you think repeated trails with samples from the same area over time would yield more conclusive data on this?

    1. we could definitely go ahead with the isolation of the old soil sample as well as a new sample and the contamination to see what results that would yield to possibly determine whether or not the corndog phage most definitely came from the contamination. I believe if we went ahead with the enrichment and isolation process of the soil sample again we would most certainly be able to conclude more accurate data. this time though through isolation of the soil sample I would throw out any spot tests with contamination and do the process again until no contamination was found.

    1. a corndog phage is a temperate siphoveridae type phage. I am not 100% sure on the rarity of the phage, however it is unable to go on to archiving as we aren’t sure whether or not this particular phage was isolated from a contamination within the lab a lot of people had a problem with.

Leave a Reply