A web pattern is when there are hundreds of plaques on a L-agar plate, making the plate very concentrated, since almost all of the bacteria has been congested by the phage, ultimately appearing as a “web pattern”.
Overall, size does not matter for bacteriophages, it only determines the morphology of the phage. For example, a phage with Myoviridae morphology will have a larger capsid head and a contractile tail, whereas if the phage had a Siphoviridae morphology will have a relatively small capsid head and a long and flexible non contractile tail. Lastly, the phage could be of Podoviridae morphology, meaning that the phage has a small capsid head and short tail.
Ultimately, only the morphology of the phage would be different, as instead of appearing as a clear plaque, it would be clear in the center with a cloudy halo surrounding it.
What is a web pattern?
A web pattern is when there are hundreds of plaques on a L-agar plate, making the plate very concentrated, since almost all of the bacteria has been congested by the phage, ultimately appearing as a “web pattern”.
Is there any further stratification to the effectiveness of various lytic phages?
Overall, size does not matter for bacteriophages, it only determines the morphology of the phage. For example, a phage with Myoviridae morphology will have a larger capsid head and a contractile tail, whereas if the phage had a Siphoviridae morphology will have a relatively small capsid head and a long and flexible non contractile tail. Lastly, the phage could be of Podoviridae morphology, meaning that the phage has a small capsid head and short tail.
would this experiment gone any differently if you used the lysogenic phage?
Ultimately, only the morphology of the phage would be different, as instead of appearing as a clear plaque, it would be clear in the center with a cloudy halo surrounding it.