10 thoughts on “P40 – Huang

  1. You said that one is most likely an O cluster phage what about the second phages’ cluster, were you able to make a hypothesis on its cluster?

    1. Thank you for your question! We were unable to determine the second phages’ cluster or even make a hypothesis because we had an overall contamination. The cluster of new phages that are isolated have to be determined through restriction digest and PCR only if it’s the phage alone. So, because we had a contamination, we weren’t able to do that. We only knew that the corndog phage was an O cluster because it’s generally known that corndog phages are almost always O cluster phages.

  2. What do you think is the most important limitation of your experiment in the context of trying to apply the results to future experiments?

    1. I appreciate your question! With our experiment, the limitation was definitely the contamination we had with the two phage species we isolated. So, when applied, the future experiments wouldn’t be as significant if we were to use these two phage species because it is expected to just use one species. Therefore, if I were to do the experiment again, I would definitely want to continue with only one isolated phage species.

  3. You said that one is most likely an O cluster phage what about the second phages’ cluster, were you able to make a hypothesis on its cluster?

    1. Thank you for your question! It was difficult to make a hypothesis on the cluster of our second phage because of our contamination. Usually, those with only one phage species would be able to do restriction digest and PCR experiments to determine cluster. However, because of our contamination, we couldn’t do that. We also only knew that the corndog phage was an O cluster because it is generally known that corndog phages are almost always in the O cluster.

    1. Hello! Thanks for your question! The overall source of the contamination is unknown. From what I’ve heard, the contamination of the corndog phage was spreading from lab section to lab section. However, it was hypothesized that contamination could’ve began when we were doing the spot titer assay and creating the plaque assays. Specifically, we think that contamination could’ve came from the top agar or the phage buffer that we were using throughout experiments.

  4. What made you want to pursue this particular lytic phage after your initial enrichment?

    1. Thank you for your question! After enrichment, we picked a plaque out of the other multiple circular plaques that formed on our plaque assay. This plaque was farther and a little more isolated from the other plaques, so it was beneficial to pick this plaque because it was less likely that this plaque region contained phages from surrounding plaques. In relation to the phage being lytic, we chose this lytic phage because it’s known that lytic phages are a little less common to isolate compared to temperate phages. Additionally, when it comes to phage therapy, lytic phages are desired over temperate phages. This interested us, so that’s why we picked it.

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