Hello, Can you please reiterate your findings in terms of phage categorization? I understand that your phage was the temperate type phage but did issues in the lab prevent you from further categorization? Good job with your presentation!
Hi! Yes, we were not able to further categorize our phage because of our issues in the lab. The main finding was that our phage is temperate based on the plaques it forms.
Hi! Enrichment increases the phage concentration in a sample through the use of LB Media. LB Media is nutritionally packed with yeast, tryptone, and sodium chloride which helps grow the bacteria and phage colonies.
That is a great question and I thought the exact same thing at first. Phage therapy is not as prevalent in our pharmacies because formulating an effective treatment is a tedious and time-consuming process. The phage first has to be isolated and proven to infect a specific bacterium before it can undergo any type of testing in vivo. Phage cocktails are also utilized which requires further experimentation. Even if the phage passes both the in-vitro and in-vivo testing stages, there is always the complication of the immune system attacking the virus which prevents it from reaching its target host cell.
Phages are not utilized by pharmacies as often as antibiotics because it is a tedious and time-consuming process to formulate an effective treatment. The phage has to be isolated and proven to infect a specific bacterium before it undergoes further in-vitro and in-vivo testing. The immune system will often attack the virus preventing it from reaching its target bacterial cell further complicating things.
Hello, Can you please reiterate your findings in terms of phage categorization? I understand that your phage was the temperate type phage but did issues in the lab prevent you from further categorization? Good job with your presentation!
Hi! Yes, we were not able to further categorize our phage because of our issues in the lab. The main finding was that our phage is temperate based on the plaques it forms.
Hi! It was cool to hear about the how phages work! How did you increase the phage concentration/what is the process of enrichment?
Hi! Enrichment increases the phage concentration in a sample through the use of LB Media. LB Media is nutritionally packed with yeast, tryptone, and sodium chloride which helps grow the bacteria and phage colonies.
Hi! Great presentation! How does the process of enrichment or increasing the phage concentration work?
Hi! Great presentation! What is the process of enrichment is and how does it works?
Since phages sound so appealing and they seem to work, why aren’t they more prevalent in our pharmacies?
That is a great question and I thought the exact same thing at first. Phage therapy is not as prevalent in our pharmacies because formulating an effective treatment is a tedious and time-consuming process. The phage first has to be isolated and proven to infect a specific bacterium before it can undergo any type of testing in vivo. Phage cocktails are also utilized which requires further experimentation. Even if the phage passes both the in-vitro and in-vivo testing stages, there is always the complication of the immune system attacking the virus which prevents it from reaching its target host cell.
Phages are not utilized by pharmacies as often as antibiotics because it is a tedious and time-consuming process to formulate an effective treatment. The phage has to be isolated and proven to infect a specific bacterium before it undergoes further in-vitro and in-vivo testing. The immune system will often attack the virus preventing it from reaching its target bacterial cell further complicating things.