12 thoughts on “P77 – Borja

  1. Which of your experiments did you think was the most interesting and/or the most useful for researching your phage?

    1. Hello Jacquie!
      I think getting the high titter lysate was definitely interesting and useful, this because I was able to ensure that I had a single specie and look at it’s behavior and morphology.

  2. Great presentation! What are the benefits of having a lytic over a lysogenic phage?

    1. Hi Kyra!
      A lytic phage helps you to destroy the bacteria cell since the phage reproduce itself inside of the bacteria, while the lysogenic the page add genetic material to the bacteria making the bacteria die but leaving the bacteria body in there. They have different applications in the case of phage therapy lytic cycles are the ones used because there’s no risk that the phage spread the DNA making the bacteria resistant to the phage.

    1. Hi! Kieran, thank you for watching!
      yes, every phage specie has different mechanisms of attaching itself to the bacteria, the most common way is that they attach its tail and contract it to injecting the DNA material inside the bacteria cell.

  3. Hi! Great work! I was just wondering if how the length of the tail and diameter of the head impact the bacteriophage? Do they make get more infectious the longer their tails are?

    1. Hi! Abbey! thanks for watching me
      The tail makes more effective the injection of the DNA in the bacteria since it contract and attach in easier way, and these specific mechanism make a phage more infectious. The specific length of the tail does not make a phage necessarily more infectious and the head of the phage impact depending in the proteins and quantity of DNA present but is not directly related to the size.

  4. How did you do the measurements using the electron microscope? Did it automatically do it for you?

  5. I was wondering how the DNA sequence would tell you more about the behavior of the phage. Would you look for specific genes that are in other phage that have proven to eliminate certain bacteria?

    1. Yes you are in the right path. Knowing the DNA sequence of Roux would helps us to predict what bacteria is efficient to kill and also some specific biological properties and behavior, these to recognize what specific DNA sequence produce certain characteristic to then be able to modify phages.

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