8 thoughts on “P45 – Randall

  1. You said your phage will be sent to a database and can later be “used as a cure for an antibacterial disease”, and I was just curious if you could elaborate on the process those researchers will use to select phages from the database? Great presentation!

    1. If our phage were to ever be sequences; which essentially tells us what our phage genome is, this is how researchers would chose our phage for treatment. However, I do not know personally how researchers would chose to sequence phages, to my understanding I think it might be random or educated guesses based on what they know about the unsequenced phage and disease.

  2. Do you think that you could expand on what the implications of understanding and characterizing bacteriophage is? Seems to be a lad unclear.

    1. Basically knowing what the phage clusters are is a way for researchers to group similar phages with similar genomes and how they function. A ‘K’ cluster phage would have different processes and genomes compared to an ‘A’ cluster phage. Knowing these characterizations allows for researchers to quickly find a phage if they are in need for a certain gene sequence.

  3. Hi! Great presentation! I was wondering how your bacteriophage that you isolated would be relevant for future research, and if your phage is successful how could it improve the market today for antibiotic resistant infections and treatment?

  4. Thank you for sharing your work. How would you decide which bacteriophage sub clusters to use in order to complete the PCR?

    1. Based on the size and type of phage(siphoviridae), we used the website phagesdb to insert this information and it provided us with the most likely subclusters to test for, which is why we tested for the 4 mentioned in the presentation. In the future if we were to come back to this experiment we would just have to do trial and error until we found the subcluster our phage belonged to.

    2. Based on the size, morphology, and that it is a siphoviridae, we inputted this data into the website phagesdb and it outputted that the most probable subclusters of our phage. So in the future is we were to continue this experiment we would need to just do trial and error until we find the correct subcluster of our phage

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