8 thoughts on “P46 – Rose

    1. This year there was an unusually high amount of corndog phages isolated throughout a lot of lab groups experiments, which is not normal. So, it was decided that any corndog phages that were isolated would not be archived this year because there is a chance that the phage we isolated was the contaminate phage!

  1. What are your expected results if you could manipulate the phage to enter at the lysogenic cycle?

    1. If we were to manipulate the phage to enter the lysogenic cycle only, you would expect that the phage would not be effective in destroying the bacterial cells. This is because phages that enter the lysogenic cylce do not immediately destroy the bacteria cell, but instead integrate their DNA into the host genome creating a prophage. If we were to manipulate the phage to only enter the lytic cycle, this would yield a higher amount of bacterial cells being destroyed because the phage in the lytic cycle will immediately lyse (kill) the host bacterial cell!

    1. In our restriction digest procedure we mix our isolated phage DNA with 6 different restriction enzymes. When we ran the gel on these 6 samples and a control group, we were able to put our results through a program called PET and the analysis showed us that O, K5, and A1 phage clusters were similar to our phages restriction digest. This allowed us to hypothesize that our phage was in one of these 3 clusters and led us to test these three cluster primers for our PCR!

  2. What are some of the ways you would able to force the phage to not enter the lysogenic cycle?

    1. The main way to prevent our phage from not entering the lysogenic cycle would be genetically modifying the DNA so the repressor protein in the phage is not produced. The repressor protein is able to bind to a special site on the DNA so genes that would cause the phage to enter the lytic cycle can not be produced, forcing the phage to enter the lysogenic cycle. By preventing the transcription of the repressor protein then all of the lytic genes can be expressed and the phage will enter the lytic cycle!

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