11 thoughts on “P51 – Jordan

  1. This was a great presentation! How would you see phage being used in the future of medicine?

    1. Hi! I’d love to look more into phage being used to treat blood infections. Due to the fact that the infections can spread so quickly through the body, it’s necessary to find a solution that works fast (lytic phage baby). If I ever do more research regarding phage, I’d love to see how this could be implemented further.

  2. Great presentation! Is there anything you could share regarding the history or origin of your phage sample?

    1. Sure! Our phage sample was collected outside of the Porter Biosciences building under a tree. Nothing too crazy which is funny considering all of our failed enrichments prior to this one. It was decently warm outside, and the dirt was wet from a recently melted snowstorm.

  3. Hi! What does it mean to have multiple species of phage? Which different species do you think you had?

    1. Hi! Essentially, there are many different mycobacteriophages. Not every mycobacteriophage is going to infect every single mycobacteria. Our sample was able to clear the plate due to having many different mycobacteriophages and thus was able to infect many of the bacteria on the plate. As far as what different species, they would all be mycobacteriophages, but I don’t think I can really specify more than that, sorry. But it would be incredibly interesting to look at this further.

  4. The presentation was really awesome! You discussed testing the adsorption rate and the host range of this specific phage in the future. What methods would you use in order to do this? Is there any previous research on this?

    1. This is a super sick question! I’d like to first address your second question. There is a TON of research regarding these two factors as the host range is important to know if you want to use the phage for phage therapy, and any other phage applications. Host range is essentially the phage’s ability to infect different bacteria. In finding host range, plating a variety of different mycobacteria and performing a plaque assay with the phage is a way to determine this. The more it’s able to lyse, the broader the host range. As far as adsorbtion rate, examining lysis time would be necessary. That is, calculating how long it takes from plating to complete lysis/lytic plaque formation.

  5. Awesome presentation! Due to PestoCraig being lytic do you think that it could be used as a form of antibiotic against specific bacteria?

    1. Hi! PestoCraig certainly has the potential to be used in treating bacterial infections. As a mycobacteriophage, PestoCraig was shown to infect nonpathogenic mycobacterium m. smegmatis. However, being able to infect m. smegmatis bacteria gives it the potential to infect pathogenic mycobacteria such as m. tuberculosis or m. leprae(which causes leprosy). Funnily enough, a phage doesn’t have to be lytic to be used in this way.

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