9 thoughts on “P54 – Hughes

  1. Hi Sara, great work on framing the purpose and conclusion of this lab! If you were to indeed “test phage against pathogenic bacteria” what you hypothesise would result? This seems like promising research!

    1. I would predict that our phage would integrate itself into the pathogenic bacteria and eliminate those bacterium without infecting natural/good bacteria.

  2. Hi Sara, great presentation. Could you explain a bit more on what is the Lytic Life Cycle and how it relates to this research?

    1. The lytic lifecycle is able to infect more cells in an organism since at the end of the cycle lytic phages lyse (are released) from the host cell. Compared to our research our phage will be able to attack more pathogenic bacteria in a shorter amount of time compared to temperate phages.

    1. Both bacteriophages are lytic and siphoviridae, meaning they will have similar characteristics on organisms.

    2. both of the bacteriophages are lytic and siphoviridae, therefore they will have similar characteristics on organisms

  3. Hey, great presentation! I really liked the way you explained why it is important to categorize the different phages. Could you explain what your expected results were at the beginning of this experiment?

    1. In the beginning of our experiment we were expecting our phage to be compromised since it took several rounds of serial dilutions to calculate our high titer lysate.

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