I liked the clear presentation of your poster and research, it felt very organized. I would love to know what the future discussions are on this poster as the video was cut off at the end.
One of the future directions would be for us to isolate DNA from our high titer lysate and then perform a quality control gel in order to determine the size of the cuts of DNA in order to then perform a restriction digest.
In image 1, you can determine that there was a phage present by the presence of plaques, or clearings on the plate. Plate 1 shows the initial presence of plaques and then in plate 2 you can guarantee that there are plaques present on your sample and that there is little contamination.
M.smeg bacteria is a part of a large family of bacteria and so when you find phages that affect M.smeg you can also apply those phages to other bacterias within the same family, such as tuberculosis.
I liked the clear presentation of your poster and research, it felt very organized. I would love to know what the future discussions are on this poster as the video was cut off at the end.
One of the future directions would be for us to isolate DNA from our high titer lysate and then perform a quality control gel in order to determine the size of the cuts of DNA in order to then perform a restriction digest.
In image 1. How do you determine that there was a phage present, and what is the difference between #1 and #2?
In image 1, you can determine that there was a phage present by the presence of plaques, or clearings on the plate. Plate 1 shows the initial presence of plaques and then in plate 2 you can guarantee that there are plaques present on your sample and that there is little contamination.
Hi Avery, great job! I was wondering if you knew what the source of contamination was?
The source of contamination was from other bacteria present in the lab. They showed up on the plates due to some sterile technique errors.
Hi Avery, great job. Do you know what the source of contamination was?
I really enjoyed your presentation! Why is Mycobaterium smegmatis more versatile than other phages?
I really enjoyed your presentation! Why is Mycobaterium smegmatis more versatile than other phages?
M.smeg bacteria is a part of a large family of bacteria and so when you find phages that affect M.smeg you can also apply those phages to other bacterias within the same family, such as tuberculosis.