8 thoughts on “P55 – Metanias

    1. Honestly, we aren’t quite sure! My guess is that because one of the two species of phage that we isolated, Clytemnestra, appeared to be decapitated in all the EM pictures of our lysate, the DNA that’s normally packaged into the capsid (head) of the phage became damaged when the head and tail were separated. This probably led to all DNA from Clytemnestra being degraded, regardless of our DNA isolation methods.

  1. What characteristics or life cycles make a phage a better candidate for antibiotic alternatives? Is the lytic cycle or lysogenic better for antibiotic use?

    1. The lytic life cycle is better for things like phage therapy, as the goal is to effectively target and infect a specific species of pathogenic bacteria. However, lytic phages aren’t necessarily going to be better candidates than temperate phages, as temperate phages can be edited into lytic phages, i.e., you can remove the genes in a temperate phage that repress the lytic cycle. Things that are more important for determining the viability of a phage for use in treatment would be things like what kind of bacteria they infect, as we want to ensure that the phage will target the pathogen without killing any beneficial bacteria in the body.

    1. Turbid is another word for cloudy, which is the kind of plaque that a temperate phage creates because it can undergo lysogeny, so it doesn’t always kill the host bacteria.

  2. Hey there! Really great job on the presentation and the poster. You did a fantastic job and it was a pleasure to watch and learn from you. The two questions I had have already been asked so I’m going to ask the the sort of annoying one (and feel free to answer it as indepth as you want to…seriously no pressure):

    If you could take this further, where would you take it? Restated: if you had a blank check and could continue your research, how would you continue it?

    1. Thank you! Honestly, if I hypothetically had unlimited funds and time to continue this research, I would probably start by going back to some of our early experiments. Continuing our research with two different species of phage isn’t really feasible, and because we don’t know when the contamination appeared, it would probably be best to go back to an early purification plaque assay and perform more purifications to try and isolate a single species. Electron microscopy would be useful to observe our lysate and see if we were successful or not. If we were, then we could move on to isolating DNA. If our DNA was of high enough quality to continue, then we could perform a restriction digest and a PCR to determine the cluster of our phage. If that’s successful, then we could archive our phage to the SEA-Phages database, and potentially have our phage sequenced to learn about its genome and its potential as a candidate for use in phage therapy.

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