8 thoughts on “P55 – Woeger

  1. Thanks for presenting!

    Is the goal to use the bacteriophage to kill bacteria (for example in an infection or as a cleaner)?

    And can the two phage you isolated work together to kill the bacteria, or do the compete with each other and one would make the other less effective?

    1. Yes, the goal is to use bacteriophage to treat harmful bacterial infections, because phage are much more specific than antibiotics, and antibiotic resistance is on the rise.

      For your second question, the phage could work together to kill the bacteria, although there would be competition for hosts. So I suppose that yes, one bacteria would me more effective at killing the bacteria faster and therefore would be able to replicate whereas the other would have issues doing so. Either way, the bacteria would die.

    1. Some long term future directions would most likely be to sequence the genome of the phage that is isolated, and then to use it in phage therapy treatments.

  2. Since this is likely a temperate bacteriophage (Clytemnestra), would it be more useful to isolate a lytic phage in order for it to hopefully infect the bacteria quicker? Or are there any advantages to this phage being temperate in terms of applicability?

    1. temperate bacteriophage can be modified to remove the repressor gene that makes it able to enter the lysogenic life cycle, so it doesn’t much matter whether the isolated bacteriophage is temperate or not. A lytic phage is preferable because it will always kill the infected bacteria within a timely manner, and we don’t want to leave any harmful bacteria within the patient’s body.

  3. Great job! Did you guys end up being able to save some of your isolated phage or was the DNA for it too degraded?

    1. No, we weren’t able to save any of our phage because we actually had 2 present in our samples. Also, the severe degradation meant that it wouldn’t be sequenced anyway.

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