8 thoughts on “P56 – Stone

    1. We used M. smegmatis as a host for our phage for a few reasons! One of the reasons is that it is nonpathogenic to humans. Another is that it is closely related to other Mycobacterium that cause leprosy and tuberculosis, and some bacteriophage that can infect M. smeg can be used in phage therapy to treat those bacteria infections.

    1. A restriction digest is a process in which DNA is cut at specific sites. We extract the phage DNA and combine it with different enzymes which will cut the phage genome in different ways, and we then run it on an agarose gel using electrophoresis. By comparing the pattern that the phage is cut in with other mycobacteriophages, we might be able to determine the cluster that the phage belongs to.

    1. As soon as we saw the phage’s EM morphology, which is very distinctive, we could visually see that it matched up with the EM of the Corndog phage on the Actinophage Database website.

  1. What other restriction digest would you use in order to get a clearer understanding of CATDOG’s genome?

    1. I’m not entirely sure what you mean by this question, but we could theoretically use different enzymes to cut the phage genome. I’m not sure that this would help us to match it to a cluster, however, as the phage on the database are mostly cut by the same few enzymes.

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