Are there any concerns about a foreign body response when you produce collagen from a human gene in a non-human host (even if it is only a soybean) and then try to place it back into a human?
What was the reason that soybeans were chosen as the model organism? Was it just the most widely available organism that could work or was it the only organism that this research could work with?
What is the difference between the alpha and beta P4H?
What is the difference between the alpha and beta PH4?
Are there any concerns about a foreign body response when you produce collagen from a human gene in a non-human host (even if it is only a soybean) and then try to place it back into a human?
What is the difference between alpha and beta PH4?
What were the proteins that helped get the P4HB into the vector? Or is it a different mechanism?
What was the reason that soybeans were chosen as the model organism? Was it just the most widely available organism that could work or was it the only organism that this research could work with?
Was there any clear causation of the offset bands in your final gel, such as contamination, uneven gel density, etc…?
Is there any other plants with plasmid manipulation potential that would be viable hosts for collagen production?