9 thoughts on “C92 – Poole

    1. This was just information that was given to us in class at the start of the semester. Past projects and experiments have investigated Rad51’s role in DNA damage likely using similar a process to the one detailed in the lab/presentation, though!

  1. What types of future experiments could be done to determine the role of Pprs42 in other pathways?

    1. First, we’d have to consider specific activities that occur during conjugation that are not related to DNA damage repair. From there, we could come with a specific hypothesis where we narrow down possible functions that Pprs42 may have. Since this was beyond the scope of the class, we didn’t go into depth about other processes that take place during this time, but considering how complex DNA replication/cell division is, I’m sure there are a lot of different possibilities!

  2. I’m wondering How you can come to the conclusion that your gene of interest is down regulated during DNA damage? I understand that your controls are present and good, but they are also going to bind at different regions.

    1. Down regulation has to do with gene expression. Our control groups were there to ensure that we didn’t make any set-up related mistakes and that there was no contamination or DNA degradation. Since it would’ve been extremely unlikely for just the DNA for our Pprs42 lanes to have been degraded (because of the other lanes produced expected results), we were able to conclude that the lack of bands was likely due to very low expression. Also, we considered that our results were similar to the consensus gels produced by other teams working on our same gene, Pprs42.

  3. I know you discussed testing primers. How did you test these primers? Moreover, what is a primer?
    Great Presentation!

    1. Thank you! A primer is just a sequence of DNA that will anneal to and isolate a specific gene sequence — in this case, we were looking for a sequence that would isolate the gene Pprs42. We did a primer validation experiment where we combined our designed primers with the genomic and coding DNA sequences of our model organism, and found that our primers did successfully anneal.

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