7 thoughts on “D104 – Hatter

  1. Hey! I really like your poster. Since you mention that in the last experiment it shows that your drug didn’t have any new salmonella growth, what would be the next steps to actually develop this drug? Also, what does bacteriostatic and bactericidal mean?

    1. Thank you so much! Our next steps would be moving out of in-vitro trials to in-vivo, using Melittin to treat mice exposed to Salmonella Typhimurium. If proven effective, and with enough support, testing in humans would follow.
      Bacteriostatic means the drug doesn’t kill bacteria, but instead prevents it from further growing/reproducing. Bactericidal means the drug kills all Salmonella bacteria, leaving none to continue growing.
      We would determine this by moving the bacteria already exposed to melittin to a new well, waiting for 24hrs, then determining whether any new growth occurred. If the bacteria was grown, then we know Melittin is bacteriostatic. If the bacteria does not grow, then it has been effectively killed and is bactericidal.
      Thanks again for your question!

  2. What is the research behind the increase in drug resistant bacteria and the reasoning for becoming more deadly than cancer in the future? I wasn’t aware of this but it is fascinating.

  3. I was surprised by the fact that drug resistant bacteria are likely to be more deadly than cancer in the next ten years. What is the reason behind this and what research supports this conclusion?

    1. Thanks for the question! As bacteria are exposed to antibiotics, all antibiotic-susceptible bacteria are killed off, leaving any mutated, antibiotic-resistant bacteria behind. With only these bacteria present, they multiply, resulting in bacterial infections antibiotics have no impact on. When our primary antibacterial defenses fail, any and all infections have the potential to grow and spread throughout the body, resulting in death. Think of any common STIs or sinus infections suddenly becoming a death sentence, all because we have no new antibiotics to combat them.
      I’ll attach a link that depicts these results, and forecasts what they mean for our future!

  4. I thought this was great! In your presentation you mentioned that you thought that you might have done some dilutions wrong because of your results. How did you confirm that you didn’t? Did you redo your experiment?

    1. Thank you! After discussing what variables could be impacting our results, we confirmed there were no errors by repeating both the max dose and dose-response curve experiments again with supervision by our TAs. It was determined to be the antibiotic success of the Melittin, which is a win for humans!

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